By Sameh Magdeldin

ISBN-10: 9535103253

ISBN-13: 9789535103257

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Example text

A part of this improvement, focused on utilization of affinity interactions in immobilization processes, will be the major part of this chapter. First part of this chapter will be aimed to the explanation of the importance of enzyme application in biotechnological processes and the improvement of their usage caused by immobilization. Advantages and limitations of immobilization processes will be described and some examples of their practical application in biotechnology and pharmaceutical industry will be mentioned.

After a second separation step, the labeled proteins are analyzed using mass spectrometry. The change in protein expression between the two cell lysates can then be quantified and related to the different conditions applied to the two sets of cell lysates. g. mass spectrometry and immunoprecipitation). One purification method which can be used to remove these contaminants either before or after other purification steps is Blue sepharose affinity chromatography. In this method, the dye ligand is covalently coupled to sepharose via a chlorotriazine ring.

One way is to insert a Affinity Chromatography: Principles and Applications 21 biotinylation sequence into a recombinant protein. Biotin protein ligase can then be used to add biotin in a post-translational modification step (Cronan & Reed, 2000). Biotin, also known as vitamin H or vitamin B7, is a relatively small cofactor present in cells. In affinity chromatography it is often used an affinity tag due to its very strong interactions with avidin and streptavidin. One advantage of using biotin as an affinity tag is that it has a minimal effect on the activity of a large biomolecule due to its small size (244 Da).

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Affinity chromatography by Sameh Magdeldin

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