By Robert B. McComb
There may be doubtless that alkaline phosphatase is among the such a lot generally in vestigated of all enzymes. This has resulted from the ubiquity of its distribution, and from the convenience and sensitivity with which its task should be measured. regrettably, those wide-ranging yet usually superficial experimental experiences were up through extensive and systematic investigations in just a number of restricted components of the biochemistry and chemical pathology of alkaline phosphatase. the end result has been the buildup of a systematic literature of intimidating proportions, and the inevitable rediscovery of already recognized proof concerning the enzyme. Scientists are taught early of their careers that, within the phrases of Sir John Herschel, "Hasty generalization is the bane of technology. " however, moments arrive in all spheres of medical job while generalization turns into crucial, to codify and to choose from the mass of knowledge already amassed, and to supply beginning issues for brand spanking new advancements and new strains of research. this can be very true in a box similar to alkaline phosphatase learn, during which very actual risks exist that the seeds of primary knowing could be misplaced amidst an unexamined harvest of empirical observations. The heritage of the examine of alkaline phosphatase offers a number of situations while priceless generalizations have emerged. sometimes, the conclusions drawn at the foundation of accessible proof have been incorrect; extra usually, they've got stood the try of extra experimentation, and continually, they've got supplied new insights into the character and correct ties of this enzyme.
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Blood phosphatase and the van den Bergh reaction in the differentiation of the several types of jaundice, Br. Med. , 1,734,1933. 171. , Serum phosphatase in jaundice, Arch. Intern. , 59, 1045, 1937. 172. Flood, C. , Gutman, E. , and Gutman, A. , Phosphatase activity, inorganic phosphorus and calcium of serum in disease of liver and biliary tract: A study of one hundred and twenty nine cases,Arch. Intern. , 59, 981,1937. 173. , Clinical significance of variations in serum phosphatase in hepatic and biliary disorders,Arch.
230. , A new phosphoric ester obtained by the aid of yeast juice; preliminary note, Proc. Chem. , 30, 16, 1914. 231. Neuman, W. , and Neuman, M. , Emerging concepts of the structure and metabolic functions of bone, Am. 1. , 22, 123, 1957. 232. Kugler, O. , Histochemical localization of alkaline phosphatase and nucleic acids in the oocytes of the bluegill (Lepomis machochirus Rajinesque), J. Histochem. , 1,296, 1953. 233. Dempsey, E. , and Wislocki, G. , Histochemical reactions associated with basophilia and acidophilia in the placenta and pituitary gland, Am.
Bounias and Pacheco 16 described alkaline phosphatase activity in young barley plants. 5, and pH optimum values were not stated. Hauck and Leithoffl7 claimed to have found some alkaline phosphatase activity in extracts prepared from Gaillardia magnifiora, but activity was low and not present in all specimens. 1 illustrates the presence of much acid phosphatase activity in extracts prepared from rye seeds. The same extracts hydrolyzed practically no phosphate esters at an alkaline pH. Butanol extraction of the insoluble residue caused no Significant alteration in pH optima.
Alkaline Phosphatase by Robert B. McComb