By Hansen, Steen; Pedersen-Bjergaard, Stig

ISBN-10: 1118716833

ISBN-13: 9781118716830

ISBN-10: 1118716868

ISBN-13: 9781118716861

ISBN-10: 1301351431

ISBN-13: 9781301351435

Show description

Read Online or Download Bioanalysis of pharmaceuticals : sample preparation, separation techniques and mass spectrometry PDF

Similar analytic books

Protein Folding, Misfolding and Aggregation

This particular ebook covers the entire glossy methods and the numerous advances skilled within the box over the last 10 years. there's a lot emphasis on computational tools and experiences of protein aggregation that have particularly flourished within the final decade. It contains chapters within the parts that experience witnessed significant advancements and written through most sensible specialists together with: laptop simulations of folding, quickly folding, unmarried molecule spectroscopy, protein layout, aggregation reports (both computational and experimental).

Separation Techniques in Clinical Chemistry

This reference examines options in separation technological know-how for more desirable sensitivity and cost-efficiency, elevated pace, greater pattern throughput and decrease solvent intake within the evaluate, assessment, and validation of rising drug compounds. It investigates breakthroughs in pattern pretreatment, HPLC, mass spectrometry, capillary electrophoresis and healing drug tracking for more desirable productiveness, precision, and defense in scientific chemistry, biomedical research, and forensic learn.

Digital Simulation in Electrochemistry

This publication is an intensive revision of the sooner publication with a similar name, 1981. The reader who has the 1st version will know chapters 1-3 and components of Chapt. four; thereafter, there's little similarity. There are numerous purposes for this. to start with, as one inner Danish ebook said, the 1st version contained "et hav af smafejl" which means (with a few poetic license), a sea of issues.

Extra info for Bioanalysis of pharmaceuticals : sample preparation, separation techniques and mass spectrometry

Sample text

When the pH is below the pI, the net charge is positive. The value of the pI can be calculated from the pKa values from all the side chains with acid–base properties as well as the pKa values of the N- and C-terminal groups. The calculation itself will not be discussed in this textbook. However, there are several web-based pI calculators available. EPO contains many functional groups with acid–base properties: 25 basic ones (3 histidine, 13 arginine, 8 lysine, and 1 N-terminal amine) and 20 acidic ones (6 aspartic acid, 13 glutamic acid, and 1 C-terminal carboxyl).

Similarly, a decrease in internal diameter of a GC capillary column will increase the efficiency. 1 Chromatographic Principles Normal Phase Chromatography Normal phase chromatography, also called straight phase chromatography, is the most common separation principle in TLC but can also be performed in HPLC mode. A polar stationary phase is used along with a more nonpolar mobile phase. A typical choice could be the use of bare silica as the stationary phase and a heptane–ethyl acetate or heptane–propanol mixture as the mobile phase.

Analytes with no affinity to (no interaction with) the polar stationary silica will not be retained and will travel with the speed of the mobile phase. Analytes having polar functional groups will have a higher affinity to the polar silica and will show retention in the system. The adsorption onto the stationary silica is a reversible interaction, and an increase in the polar component of the mobile phase will increase the competition between analyte molecules and polar molecules from the mobile phase for the adsorption sites on the surface, thus weakening the interaction of the analyte with the silica.

Download PDF sample

Bioanalysis of pharmaceuticals : sample preparation, separation techniques and mass spectrometry by Hansen, Steen; Pedersen-Bjergaard, Stig


by Michael
4.5

Rated 4.06 of 5 – based on 40 votes