By Joel Rothman, Andrew Singson

ISBN-10: 0123946204

ISBN-13: 9780123946201

The moment a part of an up-to-date variation of the vintage Methods in cellphone Biology, Volume forty eight, this e-book emphasizes assorted tools and applied sciences had to examine C. elegans, either as an built-in organism and as a version approach for learn inquiries in cellphone, developmental, and molecular biology, in addition to in genetics and pharmacology. through directing its viewers to tried-and-true and state of the art recipes for learn, this finished assortment is meant to lead investigators of C. elegans for years to come.

  • Diverse, up to date strategies coated may be important to the broadening group of C. elegans researchers for years to come
  • Chapters written by means of leaders within the field
  • Tried and real equipment carry busy researchers a one-stop compendium of crucial protocols

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Additional info for Caenorhabditis elegans : cell biology and physiology

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Biotechniques. 27, 992–996 998–1002, 1004. Piston, D. W. (1998). Choosing objective lenses: the importance of numerical aperture and magnification in digital optical microscopy. Biol. Bull. 195, 1–4. Piston, D. W. (1999). Imaging living cells and tissues by two-photon excitation microscopy. Trends Cell Biol. 9, 66–69. Piston, D. , and Knobel, S. M. (1999). Quantitative imaging of metabolism by two-photon excitation microscopy. Methods Enzymol. 307, 351–368. C. Canman (2002). Proper alignment and adjustment of the light microscope.

Housed in its eggshell, the zygote is truly an in vivo single cell system. The reproducibility of developmental events makes it possible to stage and compare fixed embryos. The early embryo is ideally suited for functional studies via protein depletion. Because of the syncytial architecture of the oogenic gonad, the preexisting pool of the target protein is actively removed and depleted to 0–5% within 24–50 h of introducing interfering RNA. Immunostaining for the target protein can roughly determine the degree of protein depletion on a single-embryo level.

Being careful not to disturb the sample or allow the coverslip to fall back on the slide, snap it off using the edge of a razor blade. 2. Fixation As you will find from the literature, there are about as many IF protocols as there are laboratories. The reader is encouraged to consult the literature and search for established protocols relating to your structure and proteins of interest, or to the specific antibody being used, if available. Once the important peculiarities of working with C. elegans embryos are addressed, the researcher can experiment with novel protocols, mixing and matching fixatives and antibodies to optimize preservation of various structures and protein epitopes.

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Caenorhabditis elegans : cell biology and physiology by Joel Rothman, Andrew Singson

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