By Paul M. Vanhoutte, Thomas Gräser, Thomas F. Lüscher (auth.), Gabor M. Rubanyi M.D., Ph.D. (eds.)

ISBN-10: 1461475147

ISBN-13: 9781461475149

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In most cases, a prominent peak of vasoconstrictor activity eluted midway between pancreatic trypsin inhibitor and cytochrome c. A selectivity curve based on the calculated partition coefficients of standard marker proteins indicated an average molecular weight of 8500 ± 1500. Although these results were very reproducible, we felt a bit uneasy about the accuracy of the determination, because preliminary experiments using ultrafiltration and dialysis membranes with defined exclusion limits had suggested that the EDCF molecule was <5000 daltons.

A. STAUDERMAN, and R. F. HIGHSMITH. Effects ofEDCF and endothelin on phosphatidylinositol hydrolysis and contraction in rat aorta. Am. J. Physiol. 258 (Cell Physiol. 27): C122-C131, 1990. 15. M. VANHOUTTE. Hypoxia releases a vasoconstrictor substance from the coronary arterial endothelium. Circulation 70: 122, 1984. 16. M. VANHOUTTE. Hypoxia releases a vasoconstrictor substance from the canine vascular endothelium. J. Physiol. ) 364: 45-56, 1986. 17. VANHOUTTE, P. M. Effects of anoxia and glucose depletion on isolated veins of the dog.

J. Physiol. 230: 1261-1268, 1976. 18. VAN NEUTEN, J. M. VANHOUTTE. Effect ofCa2 + antagonist lidoflazine on normoxic and anoxic contractions of canine coronary arterial smooth muscle. Eur. J. Pharmacal. 64: 173-176,1980. 19. , H. KURIHARA, S. KIMURA, Y. TOMOBE, M. KOBAYASHI, Y. MITSUI, Y. YAZAKI, K. GOTO, and T. MASAKI. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332: 411-415, 1988. 3 Molecular Biology of Endothelins PENNY E. PHILLIPS, CHRISTINA CADE, LYNNE H.

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Endothelin by Paul M. Vanhoutte, Thomas Gräser, Thomas F. Lüscher (auth.), Gabor M. Rubanyi M.D., Ph.D. (eds.)

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