By V. Somerset

ISBN-10: 9533074868

ISBN-13: 9789533074863

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Fumonisin mycotoxins are produced by Fusarium verticillioides and F. proliferatum species, fungi that are ubiquitous in corn (maize). Insect damage and some other environmental conditions result in the accumulation of fumonisins in corn-based products worldwide. , 2001). Both the FB1 and the OTA DNA aptamers bind to their target with nanomolar dissociation constants. 3 Heavy metals Inorganic metals contaminate foodstuffs including fish and fish products, meat and meat products, milk and milk products, eggs, fats and oils as well as animal feeds.

557-680. ; Vidal, J. ; Duato, P. & Castillo, J. An electrochemical competitive biosensor for ochratoxin A based on a DNA biotinylated aptamer. Biosensors and Bioelectronics, In Press, Corrected Proof, 0956-5663 Bruno, J. ; Carrillo, M. ; Phillips, T. & Andrews, C. J. (2010). A novel screening method for competitive FRET-aptamers applied to E. coli assay development. 1211-1223, 1573-4994; 1053-0509 Bruno, J. , & Kiel, J. L. (2002). Use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methods.

A final clean-up was then performed using solid-phase extraction (SPE) cartridges prior to analysis. Testing was performed on several spiked fish tissue samples including Salmo trutta and Oncorhynchus mykiss spp. and Salmo salar. Strong fluorescent signals were obtained in the positive samples compared to the blank controls. The threshold concentration of MG at which the signal was detectable was 5nmol/L (2ng/mL), a value 25 Advances in Aptamer-based Biosensors for Food Safety sufficient for RASFF testing standards.

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Environmental Biosensors by V. Somerset

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